The year 2009 marks the 50th anniversary of the first description of cpmv as a pathogen of cowpeas vigna unguiculata in west africa. The two most important mosaic inducing viruses of cowpea in africa are blackeye cowpea mosaic virus blcmv and cowpea aphidborne mosaic virus cabmv. Differential field infection of cowpea genotypes by southern bean mosaic virus. Cowpea yellow mosaic virus chant, 1959 cowpea mosaic virus, yellow strain agrawal, 1964 an rnacontaining virus with isometric particles about 28 nm in diameter. Relationships among serotypes of cowpea severe mosaic virus as determined by signature analysis. Relationships among serotypes of cowpea severe mosaic virus.
All structured data from the file and property namespaces is available under the. Engineering and virology of the cowpea mosaic virus and. This definition appears somewhat frequently and is found in the following acronym finder categories. Cowpea yellow mosaic virus symptoms leaf symptoms vary from green mottle to severe mosaic leaf distortion blistering and plant death control 1. Di, rong, relationships among serotypes of cowpea severe mosaic virus as determined by signature analysis 1989. General information about cowpea mosaic virus cpmsv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Tvu 2480 obtained from the international institute of. Rapid detection of cowpea aphidborne mosaic virus in cowpea seeds. Viruslike particle production using cowpea mosaic virus. Media in category cowpea mosaic virusthis category contains only the following file. As well as an intrinsic interest in cpmv as a plant pathogen, cpmv is of major interest in biotechnology applications such as nanotechnology. Its genome consists of 2 molecules of positivesense rna rna1 and rna2 which are separately encapsidated.
Selected viral families, viruses and species affected. Participation of the cowpea mosaic virus protease in. The image was created using chimera, the coordinates were downloaded from the viper database viperdb. Interference between cowpea mosaic virus and cowpea severe mosaic virus in a cowpea host immune to cowpea mosaic virus. The replication of cowpea mosaic virus, bioessays 10. Genetic inheritance of resistance to cowpea aphidborne. The first half of this dissertation chapters deals with the in vitro selfassembly of cowpea chlorotic mottle virus ccmv from its singlestranded ss rna genome and its capsid protein cp. Natural multiple viral infections of cultivated cowpeas have been reported in nigeria. The two most important mosaicinducing viruses of cowpea in africa are blackeye cowpea mosaic virus blcmv and cowpea aphidborne mosaic virus cabmv. Differences in the coat protein genes of cmv and blcmv.
Metagenomicbased screening and molecular characterization of. Research in the past 15 to 20 years has shifted from studying the underlying genetics and structure of the virus to focusing on ways in which it can be exploited in biotechnology. Cowpea mosaic virus article about cowpea mosaic virus by. Again to our surprise, all five primers gave positive results in the two samples tested. Cowpea mosaic virus cpmv was the first plant virus to be developed as a system for the display of foreign peptides and the particles have been used extensively for chemical modification.
Cowpea mosaic virus nanoparticles target surface vimentin. Cowpea mosaic virus group article about cowpea mosaic virus. Engineering cowpea mosaic virus rna2 into a vector to express heterologous proteins in plants kodetham gopinath, joan wellink,1 claudine porta, kathryn m. Genetic map of cowpea mosaic virus cpmv rna1 and representation of rna1. As well as an intrinsic interest in cpmv as a plant pathogen, cpmv is of major interest in biotechnology applications such as. Cowpea mosaic virus cpmv and flock house virus fhv are excellent model viral systems for studying and engineering these viruses. The uvvis spectrophotometry data above shows the absorbance of the virus solutions at wavelengths ranging from 230 to 700 nm. Seed transmission of cowpea aphidborne virus in some cowpea. Assessing yield potential of cowpea genotypes grown under. Frontiers additive interactions of unrelated viruses in. Cowpea aphid borne mosaic virusmorocco and south african. These positive tests not only confirmed the presence of. Scientific advances in characterising plant viruses has led to many changes in original names and bicmv is now officially bean common mosaic virus blackeye strain, but this new name is still. Lomonossoff, and ab van kammen laboratory of molecular biology, agricultural university, dreijenlaan 3, wageningen, 6703 ha, the netherlands.
As a result of its ease of propagation, high yield and the stability of the viral particles, cpmv. Relationships among serotypes of cowpea severe mosaic. Aug 15, 2011 extreme resistance of arlington line cowpea vigna unguiculata to cowpea mosaic virus cpmv is under control of a dominant locus designated cpa. Cowpea mosaic virus cpmv is a nonenveloped plant virus of the comovirus group. General information about cowpea severe mosaic virus cpsmv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Chemical modification of cowpea mosaic virus cpmv to create. Cowpea mosaic virus cpmv has become one of the most intensely studied plant viruses. Phloem loading and unloading of cowpea mosaic virus in vigna unguiculata article pdf available in journal of general virology 83pt 6. The mode of gene action conferring resistance to the virus is not well understood.
Moreover, biochemical and biophysical properties of. Sep 27, 2007 in this study, three nigerian commercial cowpea cultivars olo 11, oloyin and white and two lines from the iita it86d 719 and tvu 76 were mechanically inoculated with cowpea aphidborne mosaic virus cabmv, bean southern mosaic virus sbmv and cowpea mottle virus cmev singly, as well as in all possible combinations at 10, 20 and. Dijkstra j, bos l, bouwmeester hj, hadiastono t, lohuis h. The objective of the study was to determine the genetic inheritance of resistance in cowpea crosses. In the 50 years since it was first described, virus cpmv has become one of the most intensely studied plant viruses. Cowpea aphidborne mosaic virus cabmv is a major virus disease in uganda that causes substantial loss of the cowpea crop especially in growth and yield. Cowpea mosaic virus how is cowpea mosaic virus abbreviated. Cowpea aphidborne mosaic virus how is cowpea aphidborne mosaic virus abbreviated. About 6% of the lines, among them line arlington, showed extreme resistance as seedlings. This study reports initial optimization of the simultaneous modification of two cpmv mutants with alexafluor 647 fluorescent dyes and either igg proteins or antibodies at specific sites on the virus scaffold. We do not use these to store personal information about you. Pdf phloem loading and unloading of cowpea mosaic virus in.
Cowpea aphidborne mosaic virus how is cowpea aphid. Cowpea mosaic virus nanoparticles a structure of the cowpea mosaic virus cpmv. This study was carried out to determine the effects of single infections and coinfections of three unrelated viruses on three cowpea cultivars one commercial cowpea cultivar white and 2 iita lines. We transiently expressed, using tomato bushy stunt virus tbsv vectors and agrobacterium tumefaciens, in nearly isogenic cpacpa and cpacpa cowpea lines, sequences from rna1, the larger of two cpmv genomic rnas.
In this study, three nigerian commercial cowpea cultivars olo 11, oloyin and white and two lines from the iita it86d 719 and tvu 76 were mechanically inoculated with cowpea aphidborne mosaic virus cabmv, bean southern mosaic virus sbmv and cowpea mottle virus cmev singly, as well as in all. Some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus b1cmv while other reports have concluded. Some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus b1cmv while other reports have concluded that this. Pdf phloem loading and unloading of cowpea mosaic virus. Interactive effect of blackeye cowpea mosaic virus and cucumber. Natural spread of the cowpea strain of southern bean mosaic virus among susceptible lines and between susceptible and resistant lines. It has a limited host range, is transmitted mainly by beetles and readily by sap inoculation. Cowpea mosaic virus, sb isolate cowpea mosaic virus cpmv is a nonenveloped plant virus of the comovirus group. Infection of a susceptible cowpea leaf causes a mosaic pattern in the leaf, and results in high virus yields 12 gkg. Engineering and virology of the cowpea mosaic virus and flock. The activity of defence related enzymes peroxidase and. High performance liquid chromatography hplc profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphidborne mosaic virus cabmv. Cowpea mosaic virus cpmsv0overview eppo global database. Chemical modification of cowpea mosaic virus cpmv to.
Pdf interactive effect of blackeye cowpea mosaic virus and. More than one thousand cowpea vigna unguiculata lines were tested for resistance to cowpea mosaic virus cpmv beier et al. Protoplasts, recovered from the primary leaves of seedlings corresponding to these resistant lines and inoculated with cpmv, gave yields of cpmv. The topics include systems based on cowpea mosaic virus for the expression of antigens and antibodies in plants, a rapid system for evaluating the bioproduction capacity of complex pharmaceutical proteins in plants, chloroplastderived vaccine antigens and biopharmaceuticals, the glycosylation of antibody therapeutics, and assessing the risk that plantderived therapeutic proteins will. A veinbanding strain of cowpea aphidborne mosaic virus in nigeria. Inheritance of resistance to blackeye cowpea mosaic virus in. Sep 27, 2007 natural multiple viral infections of cultivated cowpeas have been reported in nigeria. Both the wildtype cpmv and cpmvfitc virus solutions had the same virus absorbance peaks at 260 nm. Cowpea mosaic virus cpmv has borne witness to most of the aforementioned biotechnological uses of rna viruses. A cowpea mosaic virus nanoscaffold for multiplexed antibody. Encyclopedia article about cowpea mosaic virus group by the free dictionary.
This thesis is submitted in partial fulfilment of the requirements of the degree of doctor of philosophy at the university of east anglia. The replication of cowpea mosaic virus the replication of cowpea mosaic virus van kammen, a eggen, h. Described by anderson 1955and lima et alsome isolates taiwo et al. We have systematically investigated how the assembly depends on three previously unaddressed.
A cowpea mosaic virus nanoscaffold for multiplexed. Passionfruitwoodiness virus, cowpea aphidborne mosaic virus, passiflora chlorosis virus, passiflora virus y. Both rna1 and rna2 have a vpg virus genomelinked protein at the 5end, and. Selected synonyms bean yellow mosaic virus, cowpea strain j. Molecular parameters suggest that cowpea aphidborne mosaic virus is a distinct potyvirus and bean common mosaic virus consists of at least three distinct potyviruses. Participation of the cowpea mosaic virus protease in eliciting. Presentation mode open print download current view. Cowpea mosaic virus nanoparticles target surface vimentin on. This work led first to the use of virus particles to present peptides, then to the creation of a. Oct 24, 2014 cowpea yellow mosaic virus symptoms leaf symptoms vary from green mottle to severe mosaic leaf distortion blistering and plant death control 1. In the 50 years since it was first described, cowpea mosaic virus cpmv has become one of the most intensely studied plant viruses.
Engineering cowpea mosaic virus rna2 into a vector to. Cpsmv0 cowpea severe mosaic comovirus taxonomic tree top of page. Viruslike particle production using cowpea mosaic virusbased vectors. Cowpea mosaic virus cpmv is a picornalike plant virus.
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